|Tissue standards (italicized) for vertebrate genomics corresponding to storage and quality of target materials (bolded)|
|> 700 μg||X|
|≤ 700 μg a||X|
|Cell lines/tissue culture||X|
|Live/freshly euthanized||X d||X|
aSmaller quantities (30 to 100 μg) from multiple individuals of the same species will support light-coverage sequencing for single-nucleotide polymorphism discovery.
bHigh-quality or slightly degraded DNA of small quantities will not likely be sufficient for whole-genome sequencing; these samples may supplement whole-genome sequencing efforts of higher-quality samples.
cStandards will vary depending on tissue selection and natural history of the specimen.
dFour-star samples should also include reference species for aligning de novo sequences of closely related species (see text for more details).
Wong et al. GigaScience 2012 1:8 doi:10.1186/2047-217X-1-8