Table 1 |
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| Relative cost of regenerating sequences for different classes of experiments | |||
| Class | Description | Example for DNA sequencing | Example for Imaging |
| 1 | Historical sampling of environment or time point-specific elements | Environmental genomics studies with a longitudinal component; Pathogen sequencing from epidemics | Earth imaging; environmental imaging for longitudinal studies |
| 2 | Very rare objects | Ancient DNA specimens; forensic samples | Fossils; rare meteorites |
| 3 | Longitudinal studies which could in theory be rerun in the future but have a > 10 year horizon to recreate | RNA-seq and DNA-seq from a prospective cohort; environmental sequencing of a specific field trial/intervention in an environment | MRI scans from a prospective cohort; cell imaging from a cohort |
| 4 | Samples acquired from patients or animals with a high individual acquisition cost, but a conceptually continuous generation | Cancer DNA sequencing | Histology samples from Cancer |
| 5 | A complex experiment with > 6 month resource development | RNA-seq on a specifically created mouse gene knockout (mouse colonies stored) | Cell imaging on a specific RNAi library |
| 6 | A routine experiment with < 6 month resource development | RNA-seq of a standard cell line | Routine imaging of Drosophila embryos |
| 7 | Verification experiment as a component in an overall flow | Resequencing of insert vector | Imaging of cell lines to determine confluence levels |
Relative costs decrease from class 1 through class 7.
Cochrane et al. GigaScience 2012 1:2 doi:10.1186/2047-217X-1-2
