Table 1

Relative cost of regenerating sequences for different classes of experiments
Class Description Example for DNA sequencing Example for Imaging
1 Historical sampling of environment or time point-specific elements Environmental genomics studies with a longitudinal component; Pathogen sequencing from epidemics Earth imaging; environmental imaging for longitudinal studies
2 Very rare objects Ancient DNA specimens; forensic samples Fossils; rare meteorites
3 Longitudinal studies which could in theory be rerun in the future but have a > 10 year horizon to recreate RNA-seq and DNA-seq from a prospective cohort; environmental sequencing of a specific field trial/intervention in an environment MRI scans from a prospective cohort; cell imaging from a cohort
4 Samples acquired from patients or animals with a high individual acquisition cost, but a conceptually continuous generation Cancer DNA sequencing Histology samples from Cancer
5 A complex experiment with > 6 month resource development RNA-seq on a specifically created mouse gene knockout (mouse colonies stored) Cell imaging on a specific RNAi library
6 A routine experiment with < 6 month resource development RNA-seq of a standard cell line Routine imaging of Drosophila embryos
7 Verification experiment as a component in an overall flow Resequencing of insert vector Imaging of cell lines to determine confluence levels

Relative costs decrease from class 1 through class 7.

Cochrane et al.

Cochrane et al. GigaScience 2012 1:2   doi:10.1186/2047-217X-1-2

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