|Relative cost of regenerating sequences for different classes of experiments|
|Class||Description||Example for DNA sequencing||Example for Imaging|
|1||Historical sampling of environment or time point-specific elements||Environmental genomics studies with a longitudinal component; Pathogen sequencing from epidemics||Earth imaging; environmental imaging for longitudinal studies|
|2||Very rare objects||Ancient DNA specimens; forensic samples||Fossils; rare meteorites|
|3||Longitudinal studies which could in theory be rerun in the future but have a > 10 year horizon to recreate||RNA-seq and DNA-seq from a prospective cohort; environmental sequencing of a specific field trial/intervention in an environment||MRI scans from a prospective cohort; cell imaging from a cohort|
|4||Samples acquired from patients or animals with a high individual acquisition cost, but a conceptually continuous generation||Cancer DNA sequencing||Histology samples from Cancer|
|5||A complex experiment with > 6 month resource development||RNA-seq on a specifically created mouse gene knockout (mouse colonies stored)||Cell imaging on a specific RNAi library|
|6||A routine experiment with < 6 month resource development||RNA-seq of a standard cell line||Routine imaging of Drosophila embryos|
|7||Verification experiment as a component in an overall flow||Resequencing of insert vector||Imaging of cell lines to determine confluence levels|
Relative costs decrease from class 1 through class 7.
Cochrane et al.
Cochrane et al. GigaScience 2012 1:2 doi:10.1186/2047-217X-1-2